Fetal Bovine Serum: A Crucial Growth Factor for Cell Culturing
History and Production of FBS
Fetal bovine serum (FBS) has been used extensively as a supplement in cell culture media since the mid-20th century. In the late 1940s and 1950s, scientists working to develop techniques for culturing animal cells discovered that serum obtained from blood collected from fetal calves supported cell growth better than adult bovine serum or serum from other species. Through the 1950s and 1960s, advances were made in developing standardized methods for collecting, processing, and storing FBS to maximize its growth-promoting properties and minimize variability between batches. Today, FBS production involves collecting blood from the umbilical cord of fetal calves aged 6-9 months gestation shortly after slaughter at commercial abbatoirs. The blood is allowed to clot at room temperature and the serum separated by centrifugation before being filtered, pooled, and heat inactivated at 56°C for 30 minutes to deactivate complement proteins and other heat-labile components. The processed serum is then aliquoted, cryopreserved, and sold in vials worldwide for research applications.
Components in FBS that Support Cell Growth
Fetal Bovine Serum provides crucial hormones, attachment and growth factors, lipids, minerals, and binding proteins that mimic those found in mammalian serum and support optimal cell growth, attachment, and viability in vitro. Key growth-promoting components include insulin, insulin-like growth factors I and II, fibroblast growth factor, transforming growth factor beta, epidermal growth factor, platelet-derived growth factor, and other cytokines and growth factors. FBS also contains components like fetuin, albumin, and transferrin that bind and solubilize essential growth factors and transport mineral ions important for cell metabolism.
Fetal Bovine Serum-https://www.coherentmarketinsights.com/industry-reports/fetal-bovine-serum-market
Fetal Bovine Serum: A Crucial Growth Factor for Cell Culturing
History and Production of FBS
Fetal bovine serum (FBS) has been used extensively as a supplement in cell culture media since the mid-20th century. In the late 1940s and 1950s, scientists working to develop techniques for culturing animal cells discovered that serum obtained from blood collected from fetal calves supported cell growth better than adult bovine serum or serum from other species. Through the 1950s and 1960s, advances were made in developing standardized methods for collecting, processing, and storing FBS to maximize its growth-promoting properties and minimize variability between batches. Today, FBS production involves collecting blood from the umbilical cord of fetal calves aged 6-9 months gestation shortly after slaughter at commercial abbatoirs. The blood is allowed to clot at room temperature and the serum separated by centrifugation before being filtered, pooled, and heat inactivated at 56°C for 30 minutes to deactivate complement proteins and other heat-labile components. The processed serum is then aliquoted, cryopreserved, and sold in vials worldwide for research applications.
Components in FBS that Support Cell Growth
Fetal Bovine Serum provides crucial hormones, attachment and growth factors, lipids, minerals, and binding proteins that mimic those found in mammalian serum and support optimal cell growth, attachment, and viability in vitro. Key growth-promoting components include insulin, insulin-like growth factors I and II, fibroblast growth factor, transforming growth factor beta, epidermal growth factor, platelet-derived growth factor, and other cytokines and growth factors. FBS also contains components like fetuin, albumin, and transferrin that bind and solubilize essential growth factors and transport mineral ions important for cell metabolism.
Fetal Bovine Serum-https://www.coherentmarketinsights.com/industry-reports/fetal-bovine-serum-market
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